Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for mono-layer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.
Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.
About the Author:
Martin J. Stoddart: Since 2009 Dr. Martin Stoddart has been working as a Principal Scientist at the AO Research Institute, where he is responsible for the Stem Cell Focus Area.
He completed his bachelor studies in Biology at the University of Aberystwyth in 1995. He then spent a year in Davos at ARI where he completed his M.Phil in Cartilage Biology. Between 1996-2000, he carried out his doctoral thesis at the University of Nottingham in the field of Cancer Angiogenesis. He then returned to Switzerland to work in the Laboratory for experimental cartilage research in Zurich, initially as Post-Doc and between 2003-2005 as Group Head, he also took a 6 month sabbatical at the center for Molecular Orthopedics, Harvard Medical School, Brigham and Women's Hospital, Boston, to learn viral gene transfer techniques. In 2005 he returned to ARI.
His main focus is the use of autologous stem cells and gene transfer for the repair of musculoskeletal tissues using a cell therapy approach. To this aim, he investigates novel cell identification and isolation methods. His research interests include the mechano-regulation of stem cell fate, in particular chondrogenic differentiation. He is also interested in the mechanisms by which stem cells direct cell fate and interact with endogenous cells to effect a repair.
He is the author of over 50 scientific papers, 7 book chapters and is the editor of the book, Mammalian cell Viability: Methods and Protocols. Methods in Molecular Biology-Vol.740. He is a member of the Basic Science Education Committee of the ORS. He is an Editor for Tissue Engineering Journal Parts A, B, C. He is a Scientific Editor for eCM Journal and is an organizer of the yearly eCM Conference. He is also an Editor of BioMed Research International Orthopedics and is an Associate Faculty Member of Faculty of 1000 Medicine.
About the Publisher:
Humana Press: is an academic publisher of science, technology, and medical books and journals. Humana publishes more than 100 new books and 25 journals per year, with a black list of approximately 1,500 titles in areas such as molecular biology, neuroscience, cancer research, pathology, and medicine.
The company was founded in 1976 in Clifton, New Jersey by Thomas L. Lanigan and his wife, Julia Lanigan, both chemists, and published its first book in 1977. The company was acquired by Springer Science+Business Media in the fall of 2006 and continues to publish under the Humana Press imprint. The company's employees remained at its Totowa, NJ home until June 2008, when they were moved to the Springer offices in New York City.
Following the research areas of interest of its founders, Humana publications focus the areas of molecular biology and medicine. Humana's flagship product is the Methods in Molecular Biology book series, which has produced more than 1200 published volumes, more than 33,000 individual protocols, and an extensive online database, Springer Protocols.
Publisher: Humana Press
Author: Martin J. Stoddart
Language: English
Format: PDF
Pages: 242
ISBN: 978-1-61779-108-6
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